Assessment Of Cytotoxicity Potency Of Paclitaxel In Combination With Clinacanthus Nutans Extracts On Human Mda-Mb-231 Breast Cancer Cells
- Author:
Nur Hasnieza Mohd Rosli
1
;
Chan Kok MENG
;
Fariza Juliana NORDIN
;
Lek Mun LEONG
;
Nur Syazwani Abdul Aziz
;
Nor Fadilah RAJAB
Author Information
1. Biomedical Science Programme School of Healthy Aging and Wellness (H-Care) Faculty of Health Sciences Universiti Kebangsaan Malaysia Jalan Raja Muda Abdul Aziz 50300 Kuala Lumpur, Malaysia
- Publication Type:Journal article
- Keywords:
Clinacanthus nutans;
paclitaxel;
MDA-MB-231 cells;
cytotoxicity activity
- From:Malaysian Journal of Health Sciences
2018;16(Special Issue (Article)):95-103
- CountryMalaysia
- Language:English
-
Abstract:
Clinacanthus nutans (C. nutans) leaf extracts have been widely used by cancer patients in Malaysia and local practiceclaims a cure to cancer. There were several studies done to determine the cytotoxicity potency of C. nutans extracts onvarious types of cells. However, there is still lacking on the knowledge regarding the combination effect of C. nutanswith anticancer drugs. Thus, the study was carried out to determine the cytotoxicity potency of C. nutans extracts andpaclitaxel (PTX) alone and, in combination on MDA-MB-231 cells. The cells were treated with 100% ethanol extract ofC. nutans (CNE) and water extract of C. nutans (CNA), PTX and combination of both extracts and PTX for 72 hours andthe cytotoxic activity was determined using SRB assay. Result showed that CNE had little cytotoxic activity, whereas CNAshowed no cytotoxic activity on MDA-MB-231 cells. For combination treatment of C. nutans extracts and PTX, only CNEshowed significant enhanced PTX-induced cytotoxicity (p < 0.05), meanwhile CNA inhibited PTX-induced cytotoxicitysignificantly (p < 0.05). As a conclusion, CNE was able to increase PTX potency to inhibit the viability of MDA-MB-231cells.
