Mutagenicity And Antimutagenic Activities Of Lactic Acid Bacteria (Lab) Isolated From Fermented Durian (Tempoyak)
- Author:
Asmariah AHMAD
1
;
Safura SALIK
;
Yap Wei BOON
;
Noorhisham Tan KOFLI
;
Ahmad Rohi GHAZALI
Author Information
1. Programme of Biomedical Science School of Diagnostic & Applied Health Sciences Faculty of Health Science Universiti Kebangsaan Malaysia
- Publication Type:Journal article
- Keywords:
Mutagenicity;
antimutagenicity;
Lactobacillus plantarum;
lactic acid bacteria;
Ames test;
tempoyak
- From:Malaysian Journal of Health Sciences
2018;16(Special Issue (Article)):23-26
- CountryMalaysia
- Language:English
-
Abstract:
Mutagenic and antimutagenic activities of lactic acid bacteria (LAB) Lactobacillus plantarum isolated from the localfermented durian (tempoyak) was determined by Ames test (Salmonella/microsome mutagenicity assay). Our study alsoinvolved pre-incubation assay against Salmonella typhimurium TA 98 and TA 100 bacterial strain in the presence andabsence of metabolic activator S9 system. It was found that the L. plantarum showed no mutagenic activity on bothS. typhimurium strain TA 98 and TA 100 in the presence and absence of metabolic activator. Significant antimutagenicactivity (p < 0.05) was observed in both cell-free supernatant and bacterial cell suspension of L. plantarum as comparedto the mutagenicity induced by 2-Aminoanthracene in the presence of metabolic activator. Meanwhile, in the absence ofmetabolic activator, only the bacterial cells of L. plantarum showed antimutagenicity acitivity against Sodium Azide and2-Nitrofluorene. In conclusion, L. plantarum could play a vital role as chemopreventive agent by binding to mutagensand suppressing mutagenesis. Thus, L. plantarum could be consider as a good candidate for functional food developmentas a supplement product to prevent development of colon cancer.
