Autologous mononuclear cells from different sources are seen to improve wound healing in patients with haematological malignancies
- Author:
Wan Fariza Wan Jamaludin
1
;
Farina Mohamad YUSOFF
;
Nor Azimah ISMAIL
;
Mohd Razif Mohd Idris
;
Sivakumar PALANIAPPAN
;
Christopher Ng Kee Kiat
;
Noraimy ABDULLAH
;
Seery Zaliza Azura Zaider
;
S. Fadilah S. Abdul Wahid
Author Information
1. Cell Therapy Centre, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, 56000 Kuala Lumpur, Malaysia
- Publication Type:Case Report
- Keywords:
Mononuclear cells;
autologous;
cell therapy;
wound healing;
haematological malignancies
- From:The Malaysian Journal of Pathology
2018;40(1):61-67
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Immunosuppressive state due to haematological malignancies and chemotherapy may cause disruption to wound healing despite optimum conventional treatment and standard wound dressing. Non-healing wounds are predisposed to infection whereas chemotherapy dose reductions or interruptions are associated with poor survival. Background: Mononuclear cells contain progenitor cells including haematopoietic and mesenchymal stem cells, endothelial progenitor cells and fibroblasts which facilitate wound healing through cytokines, growth factor secretions, cell-cell interactions and provision of extracellular matrix scaffolding. Clinical applications of autologous mononuclear cells therapy in wound healing in non-malignant patients with critical limb ischaemia have been reported with remarkable outcome. Methods: We report three patients with haematological malignancies undergoing chemotherapy, who received autologous mononuclear cells implantation to treat non-healing wound after optimum conventional wound care. The sources of mononuclear cells (MNC) were from bone marrow (BM), peripheral blood (PB) and mobilised PB cells (mPB-MNC) using granulocyte colony stimulating factor (G-CSF). The cells were directly implanted into wound and below epidermis. Wound sizes and adverse effects from implantation were assessed at regular intervals. Results: All patients achieved wound healing within three months following autologous mononuclear cells implantation. No implantation adverse effects were observed. Conclusions: Autologous mononuclear cells therapy is a feasible alternative to conventional wound care to promote complete healing in non-healing wounds compounded by morbid factors such as haematological malignancies, chemotherapy, diabetes mellitus (DM), infections and prolonged immobility.
