Three-Dimensional Cell Culture At The Frontiers Of In Vitro Cancer Research: Present Perspectives
- Author:
Soo Leng LEE
1
;
Zainal Ariff Bin Abdul Rahman
;
Hidetsugu TSUJIGIWA
;
Kiyofumi TAKABATAKE
;
Keisuke NAKANO
;
Wen Lin CHAI
;
Hitoshi NAGATSUKA
;
Chong Huat SIAR
Author Information
1. Department of Oral and Maxillofacial Clinical Sciences, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia
- Publication Type:Review article
- Keywords:
Cell culture;
cellular microenvironment;
cell-to-cell interaction;
cellular morphology;
cell polarity;
extracellular matrix
- From:
Annals of Dentistry
2016;23(1):1-10
- CountryMalaysia
- Language:English
-
Abstract:
In recent years, three-dimensional (3D) in vitro cell culture models have earned great attention, especiallyin the field of human cancer disease modelling research as they provide a promising alternative towardsthe conventional two-dimensional (2D) monolayer culture of cells with improved tissue organization. In2D cell culture systems, the complexity of cells on a planar surface does not accurately reflects the invivo cellular microenvironment. Cells propagated in 3D cell culture model, on the other hand, exhibitphysiologically relevant cell-to-cell interactions and cell-to-extracellular matrix (ECM) interactions,important in maintaining a normal homeostasis and specificity of tissues. This review gives an overviewon 2D models and their limitations, followed by 3D cell culture models, their advantages, drawbacks andchallenges in present perspectives. The review also highlights the dissimilarities of 2D and 3D modelsand the applicability of 3D models in current cancer research.
