Expression of Adult and Neonatal Nav1.5 Isoforms in the Dorsal Root Ganglia Neurons of Rats with Spared Nerve Injury
10.16753/j.cnki.1008-2344.2016.04.002
- VernacularTitle:成熟型与幼稚型Nav1.5 Na+通道变构体在神经损伤大鼠背根神经节细胞中的表达
- Author:
Jiatao ZHENG
1
;
Jun WANG
;
Shaowu OU
;
Yunjie WANG
Author Information
1. 中国医科大学附属第一医院神经外科
- Keywords:
sodium channel Nav1.5;
dorsal root ganglia neurons;
pain model
- From:
Journal of Shenyang Medical College
2016;18(4):229-233,236
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of adult and neonatal Nav1.5 isoforms in the dorsal root ganglia (DRG) neurons of rats with spared nerve injury (SNI) . Methods:The expression of adult and neonatal Nav1.5 isoforms in the DRG neurons of rats with SNI was detected by RT-PCR, DNA sequencing, restriction enzyme digestion, immunohistochemistry and immunofluo-rescence methods. The expression of PKC-γwas detected by Western blot. Results:Both adult and neonatal Nav1.5 isoforms were expressed in the DRG neurons, but their expression ratio was approximately 2.5∶1. In SNI rat models, the expression of both adult and neonatal Nav1.5 isoforms decreased by approximately a half in both mRNA and protein levels. In contrast, the expression of PKC-γincreased by approximately one-fold. Conclusions:Both adult and neonatal Nav1.5 isoforms expressed in the DRG neurons of rat,but their expression levels decrease in pain models. The up-regulation of PKC-γmay directly or indirectly down-regulate the expression of Nav1.5 isoforms in SNI rat models,which may further involve in the pathological process of neuropathic pain.
