Acute UV Irradiation Increases Heparan Sulfate Proteoglycan Levels in Human Skin.
10.3346/jkms.2012.27.3.300
- Author:
Ji Yong JUNG
1
;
Jang Hee OH
;
Yeon Kyung KIM
;
Mi Hee SHIN
;
Dayae LEE
;
Jin Ho CHUNG
Author Information
1. Department of Dermatology and Institute of Dermatological Science, Seoul National University College of Medicine, and Laboratory of Cutaneous Aging Research, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea. jhchung@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
- Keywords:
Acute UV Irradiation;
Human Skin;
Heparan Sulfate;
Heparan Sulfate Proteoglycan
- MeSH:
Adult;
Agrin/genetics;
Antigens, CD44/genetics;
Base Sequence;
DNA Primers/genetics;
Gene Expression/radiation effects;
Glucuronidase/genetics;
Heparan Sulfate Proteoglycans/genetics/*metabolism;
Heparitin Sulfate/metabolism;
Humans;
Male;
Matrix Metalloproteinase 1/genetics;
N-Acetylglucosaminyltransferases/genetics;
RNA, Messenger/genetics/metabolism;
Skin/*metabolism/*radiation effects;
Skin Aging/genetics/physiology;
Syndecan-1/genetics;
Syndecan-4/genetics;
Ultraviolet Rays/*adverse effects;
Young Adult
- From:Journal of Korean Medical Science
2012;27(3):300-306
- CountryRepublic of Korea
- Language:English
-
Abstract:
Glycosaminoglycans are important structural components in the skin and exist as various proteoglycan forms, except hyaluronic acid. Heparan sulfate (HS), one of the glycosaminoglycans, is composed of repeated disaccharide units, which are glucuronic acids linked to an N-acetyl-glucosamine or its sulfated forms. To investigate acute ultraviolet (UV)-induced changes of HS and HS proteoglycans (HSPGs), changes in levels of HS and several HSPGs in male human buttock skin were examined by immunohistochemistry and real-time quantitative polymerase chain reaction (qPCR) after 2 minimal erythema doses (MED) of UV irradiation (each n = 4-7). HS staining revealed that 2 MED of UV irradiation increased its expression, and staining for perlecan, syndecan-1, syndecan-4, CD44v3, and CD44 showed that UV irradiation increased their protein levels. However, analysis by real-time qPCR showed that UV irradiation did not change mRNA levels of CD44 and agrin, and decreased perlecan and syndecan-4 mRNA levels, while increased syndecan-1 mRNA level. As HS-synthesizing or -degrading enzymes, exostosin-1 and heparanase mRNA levels were increased, but exostosin-2 was decreased by UV irradiation. UV-induced matrix metalloproteinase-1 expression was confirmed for proper experimental conditions. Acute UV irradiation increases HS and HSPG levels in human skin, but their increase may not be mediated through their transcriptional regulation.
