Therapeutic Effect of Hyaluronic Acid Chitosan-based Microemulsion and Carboplatin on Glioma in Rats
10.16753/j.cnki.1008-2344.2016.03.007
- VernacularTitle:类透明质酸壳聚糖微乳和碳铂联合给药对大鼠胶质瘤的治疗效果评价
- Author:
Weicheng LU
1
;
Hui XIE
;
Xiaohui DING
;
Rong QI
;
Haihui XING
;
Haopeng WU
Author Information
1. 中国医科大学附属第一医院神经外科
- Keywords:
hyaluronic acid chitosan-based microemulsion;
glioma;
carboplatin;
survival time
- From:
Journal of Shenyang Medical College
2016;18(3):149-150,153
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the therapeutic effect of hyaluronic acid chitosan?based microemulsion ( HAC?ME) and carboplatin in a Wistar rat model bearing cerebral C6 glioma xenografts. Methods:C6 rat glioma cells were cultured normally. A total of 30 Wistar rats bearing orthotopic C6 glioma xenografts were randomly divided into 3 groups, and administrated with physiological saline, carbopl?atin or HAC?ME and carboplatin in each group. There are 10 rats in each group. The rat apoptosis changes and survival time were ob?served after treated with physiological saline, carboplatin or HAC?ME and carboplatin in each group. Results:Glioma cells were nega?tive in saline group with TUNEL staining, the nuclei of individual glioma cells in glioma tissue were stained with brown, indicating ap?optosis occur in carboplatin group, the apoptosis of glioma cells in glioma tissue significantly increased in HAC?ME and carboplatin group with TUNEL staining. The survival time in HAC?ME and carboplatin group was longer than that in saline group and carboplatin group ( P<0?05) . Conclusion:Administration of HAC?ME and carboplatin can suppress the growth of C6 glioma in rats and may pro?vide experimental basis for clinical treatment of glioma.
