Detecting the Expression of ST3GalⅤ in Human Hepatic Carcinoma Cell Lines by Sialyltransferase DNA Microarray
10.16753/j.cnki.1008-2344.2016.03.005
- VernacularTitle:应用唾液酸糖基转移酶基因芯片检测ST3GalⅤ在人肝癌细胞系中的表达
- Author:
Yan ZHANG
1
;
Hao FU
;
Yan GANG
;
Yujie ZHAO
;
Qun HE
Author Information
1. 沈阳医学院基础医学院生物化学教研室
- Keywords:
DNA microarray;
sialyltransferase;
ST3GalⅤ;
hepatic carcinoma
- From:
Journal of Shenyang Medical College
2016;18(3):142-145
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the differential expression of ST3GalⅤ in human hepatic carcinoma cell lines, HepG?2 and SMMC?7721 and normal hepatic cell line, L?02 by sialyltransferase DNA microarray. Methods: Expression of ST3Gal family was measured with DNA microarray. Western blot was used to verify DNA microarrays data. Result:ST3GalⅤ downregulated in HepG?2 and SMMC?7721 cell lines, compared with control cell line. Conclusion: The downregulation of ST3GalⅤ in HepG?2 and SMMC?7721 cell lines may result in the reduction of GD1a and influence the hepatocyte proliferations.
