Influence of IKK Inhibitor on Carotid Artery Restenosis in Rat by Balloon Catheter Injury
10.16753/j.cnki.1008-2344.2016.03.004
- VernacularTitle:IKK抑制剂对大鼠颈动脉球囊损伤术后再狭窄的影响
- Author:
Guiping WU
1
;
Wei CAO
;
Yanhong ZHANG
;
Ying ZHOU
;
Huiming DENG
;
Jun XING
Author Information
1. 沈阳医学院附属第二医院心内科
- Keywords:
IKK;
IKK inhibitor;
restenosis;
nuclear factor kappa B
- From:
Journal of Shenyang Medical College
2016;18(3):138-141
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of inhibitor of kappa B kinase ( IKK) inhibitor on carotid artery restenosis in rat by balloon catheter injury. Methods: A total of 32 male Wistar rats were randomly divided into control group, injured group and med?icine group. The balloon catheter injury was performed on left common carotid artery of rat by imitating the process of angioplasty. In?jured group and control group were injected saline, medicine group were injected IKK inhibitor. After 15 days, the process of neointi?mal and media hyperplasia was observed by HE staining and the expression level of NF?κB, IKK, inhibitor of nuclear factor kappa B ( IκB) was detected by Western blot, the content of IL?6, TNF?αwas detected by radioimmunoassay, and the content of MMP?1 was determined by MMP?1 kit. Results: Arterial neointima hyperplasia hapened in 3 groups. There was significantly difference between control group and medicine group and injured group in intimal area, the expression level of NF?κB, IKK, IκB, and the content of IL?6, TNF?α and MMP?1 ( P<0?05 or 0?01) . There was no significance in these indexes between control group and medicine group ( P>0?05) . Conclusion:IKK inhibitor can reduce the carotid artery restenosis after balloon injury by inhibiting inflammatory reaction induced by IKK?IκB?NF?κB pathway.
