Summary of experience in the establishment of rat models with orthotopic liver transplantation from donation after cardiac death
10.3969/j.issn.1674-7445.2018.04.011
- VernacularTitle:大鼠心脏死亡器官捐献供体原位肝移植模型建立的经验总结
- Author:
Ruichao WU
1
;
Zhaoyu HUANG
;
Li ZHANG
;
Junhan LIU
;
Kepu ZHENG
;
Jianghua RAN
Author Information
1. 650500,昆明医科大学附属甘美医院暨昆明市第一人民医院
- Keywords:
Donation after cardiac death;
Donor;
Orthotopic liver transplantation;
Rat model;
Warm ischemic time;
Anhepatic phase;
Biliary complication;
Hepatic failure
- From:
Organ Transplantation
2018;9(4):304-310
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the skills and summarize the experience in the establishment of orthotopic liver transplantation rat models from donation after cardiac death (DCD). Methods According to the time of warm ischemia, 120 rats were divided into 3 groups: group A (warm ischemia for 0 min, n=40 pairs), group B (warm ischemia for 10 min, n=40 pairs) and group C (warm ischemia for 20 min, n=40 pairs). Orthotopic liver transplantation was performed by the modified two-cuff technique in 3 groups. The time of each stage of surgery was recorded in 3 groups. The survival rate at the end of surgery, 24 h, 72 h and 7 d after surgery was recorded in 3 groups. The dead rats were immediately subject to anatomical examination to identify the cause of death. Results The cold ischemia time of donor liver, anhepatic phase and operation time of the recipients did not significantly differ among three groups (all P>0.05). In groups A, B and C, the survival rate at the end of surgery was 97%, 97%, and 100% respectively. The survival rate at postoperative 24 h was 92%, 90% and 92% respectively. The survival rate at postoperative 72 h was 90%, 80% and 77% respectively. The survival rate at postoperative 7 d was 85%, 70% and 57% respectively. The survival rate at the end of surgery, postoperative 24 h and 72 h did not significantly differ among 3 groups (all P>0.05). At postoperative 7 d, the survival rate in group C was significantly lower than that in group A (P<0.05). Surgical operation was the major cause of intraoperative and postoperative 24 h death. Bile leakage and ischemic hepatic failure were the causes of death at postoperative 72 h. Biliary duct complications were the main causes of death at postoperative 7 d. The quantity of rats developing with biliary duct complications was increased along with the prolongation of warm ischemic time. Conclusions The success of stable establishment of rat models with orthotopic liver transplantation from DCD depends upon the protection of the liver and biliary function. The difficulty lies in the anastomosis of the suprahepatic inferior vena cava and the shortening of anhepatic phase.
