High-throughput sequencing analysis of single nucleotide polymorphism loci mutation in genes associated with acute rejection in liver transplantation
10.3969/j.issn.1674-7445.2018.03.007
- VernacularTitle:高通量测序分析肝移植急性排斥反应相关基因单核苷酸多态性位点的突变
- Author:
Yuanda BAO
1
;
Shuhong YI
Author Information
1. 中山大学附属第三医院肝脏外科暨肝移植中心
- Keywords:
Liver transplantation;
Acute rejection;
High-throughput sequencing;
Single nucleotide polymorphism (SNP);
Interleukin;
Cell chemokine receptor;
Nuclear factor-kappa B inhibitor alpha;
Vitamin D receptor;
Gene mutation
- From:
Organ Transplantation
2018;9(3):205-210
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the mutation of single nucleotide polymorphism (SNP) loci in genes most significantly associated with acute rejection after liver transplantation by high-throughput sequencing. Methods Peripheral blood samples were collected from 68 recipients undergoing allogeneic orthotopic liver transplantation. According to the incidence of acute rejection, all patients were divided into the acute rejection group (n=13) and non-rejection group (n=55). Through the literature review, 44 mutant SNP loci associated with acute rejection were finally identified. Using 44 SNP loci as the detection targets, high-throughput sequencing analysis was performed to detect peripheral blood samples in two groups of recipients. Bioinformatics analysis revealed the mutation rate of the SNP loci of genes related to acute rejection. Results The mutation rate of SNP loci of the interleukin(IL)-10 TT genotype,T allele,AA genotype and A allele in the acute rejection group was significantly higher than that in the non-rejection group. The mutation rate of the SNP loci of the cell chemokine receptor(CCR)5 AG genotype in the acute rejection group was significantly lower than that in the non-rejection group,the mutation rate of the SNP loci of the CCR5 GG genotype in the acute rejection group was significantly higher than that in the non-rejection group.The mutation rate of the SNP loci of IL-4 CT genotype in the acute rejection group was significantly higher than that in the non-rejection group,the mutation rate of the SNP loci of IL-4 TT genotype in the acute rejection group was significantly lower than that in the non-rejection group. The mutation rate of the SNP loci of nuclear factor-kappa B inhibitor alpha(NF-κBIA)C allele in the acute rejection group was significantly higher than that in the non-rejection group.The mutation rate of the SNP loci of vitamin D receptor(VDR)CC genotype and C allele in the acute rejection group was significantly lower than that in the non-rejection group. Differences were statistically significant (all P<0.05). Conclusions High-throughput sequencing analysis shows the genes associated with acute rejection after liver transplantation.Among them,the mutation rate of the SNP loci is relatively high in IL-10 TT genotype,T allele, AA genotype,A allele,CCR5 GG genotype,AG genotype,IL-4 CT genotype,TT genotype,NF-κBIA C allele,VDR CC genotype and C allele.