Effect of extracorporeal photopheresis on the expression of IL-12p70 and Th1/Th2-like cytokines in mouse models with skin allograft
10.3969/j.issn.1674-7445.2017.05.004
- VernacularTitle:体外光分离置换疗法对皮肤移植小鼠IL-12p70和Th1/Th2类细胞因子产生的影响
- Author:
Yuxiang WEI
1
;
Li XIAO
;
Ming CAI
;
Yeyong QIAN
;
Tao YU
;
Bingyi SHI
Author Information
1. 解放军第309医院全军器官移植研究所 北京市器官移植与免疫调节重点实验室
- Keywords:
Extracorporeal photopheresis;
Mouse skin transplantation;
Interleukin;
T helper cell (Th) 1/Th2;
8-methoxypsoralen;
Apoptosis;
Immune regulation;
Rejection reaction
- From:
Organ Transplantation
2017;8(5):355-359
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of extracorporeal photopheresis upon the expression levels of interleukin (IL)-12p70 and T helper cell (Th) 1/Th2-like cytokines in splenic lymphocytes of mouse models undergoing skin transplantation. Methods The C57BL/6 mice were used as the donors and BALB/c mice as the recipients to establish mouse models with skin allograft. The splenic lymphocytes in the C57BL/6 and BALB/c mice (CSP and BSP) were isolated and treated with 8-methoxypsoralen combined long-wave ultraviolet (PUVA-SP). According to the components of intravenous infusion into the recipients, all experimental animals were randomly divided into the PUVA-BSP, PUVA-CSP, BSP, CSP and phosphate buffer solution (PBS) control groups (n=12 for each group). The mice were injected with PUVA-BSP, PUVA-CSP, BSP, CSP or PBS via the caudal vein at preoperative 7 d, upon the day of surgery and at postoperative 7 d, respectively. The apoptosis of the splenic lymphocytes was observed after PUVA treatment. The expression levels of IL-12p70 and Th1/Th2-like cytokines in the peripheral blood of the recipients were quantitatively measured. Results After the skin transplantation, the expression levels of IL-12p70 in the peripheral blood of mice in the PUVA-BSP and PUVA-CSP groups were significantly down-regulated compared with those in the BSP, CSP and PBS control groups (all P<0.01). In the PUVA-BSP and PUVA-CSP groups, the expression levels of Th1-like cytokine IL-2, interferon (IFN)-γwere dramatically lower than those in the BSP, CSP and PBS control groups (all P<0.01). The expression levels of Th2-like cytokine IL-10 in the PUVA-BSP and PUVA-CSP groups were significantly up-regulated compared with those in the BSP, CSP and PBS control groups (all P<0.01). Conclusions Infusion of PUVA-SP at a sufficient dose can induce the low expression level of IL-12p70 and drive the incidence of Th2 immune deviation in the recipient BALB/c mice.
