Study on variation characteristics of anti-donor specific antibodies in different species of sensitized mice after skin transplantation
10.3969/j.issn.1674-7445.2017.03.006
- VernacularTitle:不同品系小鼠间皮肤移植致敏后抗供体特异性抗体的变化特点研究
- Author:
Tao LIAO
1
;
Daqiang ZHAO
;
Siwen LI
;
Fei HAN
;
Zihuan LUO
;
Xiaonan LIU
;
Qiquan SUN
Author Information
1. 中山大学附属第三医院肾移植科
- Keywords:
Skin transplantation;
Donor-specific antibody (DSA);
Antibody-mediated rejection (AMR);
Mouse;
Immune response;
Renal transplantation;
Animal model;
Immunoglobulin (Ig)
- From:
Organ Transplantation
2017;8(3):205-208
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the change features of anti-donor specific antibody (DSA) in different species of sentitized mice after skin transplantation. Methods All mice were divided into the Balb/c → C57BL/6 (6 pairs) and Balb/c → C3H skin transplantation groups (6 pairs). At d0, d2, d4, d7, d13, d17, d28, d35, d42, d49 and d56 after skin transplantation, the serum sample was prepared for detection of DSA-IgG and DSA-IgM levels. Results Moderate increase was noted in the DSA-IgG level in the sensitized mice within 1 week after skin transplantation. The IgG level was significantly increased within 1-4 week and peaked and stabilized within 4-8 week. No significant variation was observed in the DSA-IgM level at 8 weeks after skin transplantation. In the Balb/c → C57BL/6 skin transplantation group, the DSAIgG level was significantly lower than that in the Balb/c → C3H group. Statistical significance was identified in the IgG levels between two groups at d2, d17, d28, d35, d42, d49 and d56 after skin transplantation (all P<0.05). No statistical significance was noted in the DSA-IgM levels between two groups at each time point (all P>0.05). Conclusions Advancing the time of renal transplantation after skin transplantation moderately in the Balb/c → C3H group, or changing to the lower immunoreactive combination of Balb/c → C57BL/6 are aimed to establish AMR mouse models with mild rejection reaction.
