Activation of CD8+T cells regulated by γ-aminobutyric acid and its receptors
10.3969/j.issn.1674-7445.2017.02.010
- VernacularTitle:γ-氨基丁酸及其受体调控CD8+T细胞活化
- Author:
Wenmei FAN
1
;
Yu GAO
;
Yujie SUN
;
Xihui MA
;
Xiuyun HE
;
Li XIAO
;
Bingyi SHI
Author Information
1. 解放军第309医院全军器官移植研究所研究室
- Keywords:
CD8+T cell;
CD3/CD28;
γ-aminobutyric acid;
Transplantation immunity;
GABA-A receptor;
GABA-B receptor;
Cell proliferation;
T cell activation;
CD152
- From:
Organ Transplantation
2017;8(2):144-148
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of γ-aminobutyric acid (GABA) and its receptors upon the proliferation of CD8+T cells.Methods The splenic CD8+T cells of Balb/c mice were obtained by CD8+f cell magnetic bead sorting kit.Under the effect of CD3/CD28-activated magnetic bead,CD8+T cells were stimulated by different concentrations of GABA.5-bromo-2-deoxyuridine (BrdU) labeling and flow cytometry were performed to detect the proliferation of CD8+T cells.The expression levels of GABA-A and GABA-B receptor before and after CD8+T cell activation were compared by fluorescent quantitative real-time polymerase chain reaction (PCR).Result Flow cytometry result revealed that GABA could inhibit the proliferation of activated CD8+T cells,manifested as significant decrease in the quantity of CD152+CD8+T cells.Fluorescent quantitative real-time PCR demonstrated that GABA-A receptor subtypes α2,α6 and GABA-B receptor subtype 1a were expressed only when the CD8+T cells were activated.After CD8+T cell activation,the quantity of GABA-A receptor subtypes α3,αs,β2,β3,γ1,γ2 and θ were significantly increased,whereas the quantity of GABA-B2R and GABA-B1b did not significantly differ before and after CD8+T cell activation.Conclusions GABA can suppress the proliferation of activated CD8+T cells.The activation of CD8+T cells is regulated by GABA receptors.However,the underlying mechanism remains to be elucidated.
