Breeding and identification of Wuzhishan miniature pigs with α-1,3-galactosyltransferase gene-knockout
10.3969/j.issn.1674-7445.2017.02.006
- VernacularTitle:敲除α-1,3-半乳糖基转移酶基因五指山小型猪的繁育和鉴定
- Author:
Chuan LONG
1
,
2
;
Jingbo GAO
;
Yuting TANG
;
Minjie DU
;
Ningning SHI
;
Chong FENG
;
Lin LU
;
Dengke PAN
Author Information
1. 100193 北京,中国农业科学院北京畜牧兽医研究所
2. 北京农学院动物科学技术学院
- Keywords:
α-1,3 galactosyltransferase;
Gene knockout;
Wuzhishan miniature pig;
Reproduction performance;
α-1,3 galactosyl phenotype;
Peripheral blood mononuclear cell
- From:
Organ Transplantation
2017;8(2):121-126
- CountryChina
- Language:Chinese
-
Abstract:
To summarize the breeding and identification of Wuzhishan miniature pig models with α-l,3-galactosyltransferase (GGTA1) gene-knockout (GTKO).Methods The breeding and reproduction perform of GTKO Wuzhishan miniature pigs were assessed and the quantity of piglets was counted.The GTKO Wuzhishan miniature pig models with GGTA1gene knockout were validated by polymerase chain reaction(PCR).The αGal phenotype of peripheral blood mononuclear cells (PBMC) in human,wild-type Wuzhishan miniature pigs and GTKO Wuzhishan miniature pigs was detected by fluorescent microscope and flow cytometry.Routine blood test parameters were statistically compared between the GTKO and wild-type Wuzhishan miniature pigs.Results The inheritance of GGTA1 genotype complied with Mendel's law.Flow cytometry detected no fluorescent expression of PBMC in GGTA1-/-pig models,which were consistent with the genotype identification results.The mean piglets of the primiparous GTKO Wuzhishan miniature pigs were (6.8±1.8) and (8.3±2.2) for the multiparous Wuzhishan miniature pigs.No statistical significance was noted in routine blood test parameters between the GTKO and wild-type Wuzhishan miniature pigs (all P＞0.05).Conclusions Stable inheritance and normal reproductive capacity are observed in two generations of Wuzhishan miniature pigs continuously.GTKO Wuzhishan miniature pig is a reliable donor for heterogeneous organ transplantation.
