Changes of renal resident dendritic cells during kidney ischemia-reperfusion injury
10.3969/j.issn.1674-7445.2016.05.006
- VernacularTitle:肾脏固有树突状细胞在肾缺血-再灌注期间的变化
- Author:
Chen YAO
1
;
Shuxin LI
;
Tao YU
;
Xiaodong XU
;
Bingyi SHI
Author Information
1. 解放军第309医院全军器官移植研究所 北京市器官移植与免疫调节重点实验室
- Keywords:
Kidney;
Dendritic cell;
Ischemia-reperfusion injury;
Mouse;
Flow cytometry
- From:
Organ Transplantation
2016;7(5):360-364
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of renal resident dendritic cells (rDC)during kidney ischemia-reperfusion injury (IRI). Methods C57BL/6J mice models with bilateral renal warm ischemia were established. The kidney tissue was prepared for single cell suspension at 24 h and 48 h after reperfusion. The changes in the percentage of CD45 +cells and CD1 1 c +rDCs were evaluated by flow cytometry. The renal tissues of mice labeled with green fluorescent protein and diphtheria toxin receptor (CD1 1 c +GDTR ) were prepared for single cell suspension. The percentage and phenotype of CD1 1 c +rDCs were analyzed by flow cytometry. CD1 1 c +GDTR mice models with bilateral renal warm ischemia were established. The renal tissue was prepared for single cell suspension at 24 h after reperfusion. CD45 + cells was gathered by magnetic-activated cell separation (MACS ). The expression levels of co-stimulatory molecules on the rDC surface were analyzed by flow cytometry. Results At 24 h after reperfusion,the percentage of CD45 +cells in the kidney of C57BL/6J mice was significantly elevated,and further increased at 48 h after reperfusion. At 24 h after reperfusion,the quantity of CD1 1 c +rDCs was equally increased,whereas the percentage of CD1 1 c +rDCs in CD45 +cells was dramatically declined and restored at 48 h after reperfusion,slightly higher compared with that in the sham group. In healthy CD1 1 c +GDTR mice,the percentage of CD45 +cells in the kidney was lower than 1%,consisting of approximately 40%of CD1 1 c +rDCs,which mainly presented as CD11bintF4/80 -MHCⅡ+. At 24 h after reperfusion,the percentage of CD11c +F4/80 -subset rDC surface co-stimulatory molecules was significantly enhanced,such as CD40,CD80 and CD86. Conclusions Following warm IRI,the percentage and quantity of rDCs,and the expression level of rDC surface co-stimulatory molecule are significantly increased,prompting that renal rDC infiltration is increased and phenotype becomes matured.
