Lienal polypeptide injection attenuates lipopolysaccharide-induced acute lung injury in rats by metastasis-associated protein 1
10.7507/1007-4848.201803045
- VernacularTitle:脾多肽注射液通过肿瘤转移相关蛋白 1 参与大鼠急性肺损伤的保护作用
- Author:
GE Peng
1
,
2
,
3
;
LI Hanjie
1
,
2
,
3
;
CHEN Xin
1
,
2
,
3
;
JING Ruijun
1
,
2
,
3
;
YAO Yuejuan
1
,
2
,
3
;
LI Jianzhong
4
,
5
,
6
;
ZHANG Weidong
7
;
YANG Bo
7
Author Information
1. Department of Cardio-Thoracic Surgery, Second Affiliated Hospital, Xi'
2. an Medical College, Xi'
3. an, 710038, P.R.China
4. Department of Thoracic Surgery, Second Affiliated Hospital of Xi'
5. an Jiaotong University, Xi'
6. an, 710004, P.R.China
7. Department of Thoracic Surgery, Tianjin First Central Hospital of Nankai University, Tianjin, 300192, P.R.China
- Publication Type:Journal Article
- Keywords:
Lienal polypeptide injection;
lipopolysaccharide;
acute lung injury;
metastasis-associated protein 1
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2019;26(3):264-268
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the role of lienal polypeptide injection in acute lung injury induced by lipopolysaccharide (LPS) in rats. Methods Eighty male SD rats were randomly allocated into 4 groups: a LPS group, a control group, a lienal polypeptide group and a LPS+ lienal polypeptide group (20 rats in each group). Lienal polypeptide or normal saline was given with an intramuscular injection 30 min after an intraperitoneal injection of LPS (5 mg/kg). The severity of pulmonary injury was evaluated 4 h after LPS challenge by enzyme-linked immunosorbent assay (ELISA), wet-to-dry weight ratio, hematoxylin and eosin (HE) staining, TUNEL and Western blotting. Results Lienal polypeptide injection treatment significantly attenuated LPS-induced pulmonary histopathologic changes, alveolar hemorrhage, and neutrophil infiltration. Moreover lienal polypeptide injection significantly suppressed LPS-induced activation of metastasis-associated protein-1 (MTA1). Conclusion Lienal polypeptide injection is demonstrated to protect rats from LPS-induced acute lung injury by the expression of MTA1.
