The creation of the reconstruction of right ventricular outflow tract in rats
10.7507/1007-4848.201811045
- VernacularTitle:大鼠右室流出道重建术模型的构建
- Author:
CHENG Yue
1
;
KANG Kai
1
;
CHUAI Junbo
1
;
QIN Xionghai
1
;
TIAN Xin
1
;
YANG Feng
1
;
JIANG Shulin
1
;
XIE Baodong
1
Author Information
1. Department of Cardiovascular Surgery, 2nd Affiliated Hospital of Harbin Medical University, Key Laboratory of Myocardial Ischemia Mechanism and Treatment, Ministry of Education, Harbin, 150086, P.R.China
- Publication Type:Journal Article
- Keywords:
Right ventricular outflow tract;
reconstruction;
rat;
cardiac patch;
tissue engineering
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2019;26(3):260-263
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of animal model of the reconstruction of right ventricular outflow tract in rats. Methods A total of 15 female Sprague-Dawley (SD) rats underwent right ventricular outflow tract reconstruction surgery. Before the operation, the collagen scaffolds were treated with g 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride chemistry (EDC), and seeded with human bone marrow stem cells (h-MSCs). Three days after the surgery, 3 rats were randomly sacrificed to evaluate the transmural resection of right ventricular outflow tract. One or 3 months later, other 3 rats at each timepoint were sacrificed, stained with Masson’s Trichrome to observe the degradation of scaffold. Furthermore, 4 weeks after the surgery, 4 rats were sacrificed and the hearts were sliced. Anti-human mitochondria staining was used to identify the survival of seeding cells. Results The transmural resection of right ventricular outflow tract was feasible in rats at an acceptable mortality (13.3%). After EDC treatment, the degradation rate of collagen scaffold was extended greatly. The seeding cells were detected by anti-mitochandria immunofluorescent staining in all patches 4 weeks after the operation. Conclusion Rat model of right ventricular outflow tract reconstruction could be a stable, reliable and economical screening model for engineered heart tissue research.
