Expression and significance of 8-OHdG in primary pterygium
- VernacularTitle:8-羟基脱氧鸟苷在原发性翼状胬肉组织中的表达及意义
- Author:
Huai-Yun Jin
1
Author Information
- Publication Type:Journal Article
- Keywords: pterygium; 8-hydroxy-deoxyguanosine; oxidative DNA damage
- From: International Eye Science 2017;17(3):565-567
- CountryChina
- Language:Chinese
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Abstract:
AIM:To detect oxidative DNA damage marker 8-hydroxy-2-deoxyguanosine(8-OHdG)in primary pterygium and normal conjunctival tissues, explore the role of oxidative DNA damage in the pathogenesis of pterygium.
METHODS: Totally 35 primary pterygium specimens were collected during surgery and 5 normal conjunctival specimens which above the normal temporal bulbar conjunctiva were collected. The expressions of 8-OHdG in pterygium tissues were detected by immunohistochemical method and compared with the normal conjunctival tissues. The difference of 8-OHdG expression between the two groups was compared.
RESULTS: There were 24(69%)pterygium specimens positive for 8-OHdG staining, limited to the nuclei of the epithelial layer. No substantial staining was visible in the subepithelial fibrovascular layers. All normal controls were negative for 8-OHdG staining. The difference of 8-OHdG expression between the two groups was statistically significant(P=0.007).
CONCLUSION: The increased levels of 8-OHdG in the pterygium tissues indicate that oxidative DNA damage maybe play an important role in the pathogenesis of pterygium.
