Establishment of rat bone mesenchymal stem cells expressing IL-33
- VernacularTitle:重组慢病毒GV303 Rat IL-33体外转染原代大鼠骨髓间充质干细胞的研究
- Author:
Xue-Yan Yao
1
Author Information
- Publication Type:Journal Article
- Keywords: Interleukin-33; transfect; bone mesenchymal stem cells; experimental autoimmue uveitis
- From: International Eye Science 2017;17(5):841-845
- CountryChina
- Language:Chinese
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Abstract:
AIM: To explore a way to establish a rat bone mesenchymal stem cell(BMSC)line expressing Interleukin(IL)-33.
METHODS: BMSCs were isolated from 6-week-old Wistar Rat. GV303 virus were used as a IL-33 gene carrier to tansfect isolated BMSCs.Three days later, transfected BMSCs were observed under a fluorescence microscope. The expression level of IL-33 of transfected BMSCs was detected by Western blot and ELISA.
RESULTS: The BMSCs were successfully isolated, since flow cytometry results showed that rat BMSCs CD90 and CD29 positive, CD45,CD34 and CD11b negtive. Furthermore, BMSCs were able to be differentiated to osteoblasts, adipocytes and chondrocytes respectively. Green fluorescence of GFP BMSCs was observed under the fluorescence microscope 3d after virus transfecting. Expression of IL-33 was detected by Western blot and ELISA in the transfected rat BMSCs.
CONCLUSION: A rat BMSC cell line expressing IL-33 was established by GV303 virus transfection.
