Hypoxia induces Wee1 expression and attenuates hydrogen peroxide-induced endothelial damage in MS1 cells.
10.3858/emm.2011.43.12.074
- Author:
Ki Sun HONG
1
;
Hyeon Soo KIM
;
Se Hoon KIM
;
Dong Jun LIM
;
Jung Yul PARK
;
Sang Dae KIM
Author Information
1. Department of Neurosurgery, Korea University Ansan Hospital, Korea University College of Medicine, Ansan 425-707, Korea. neuron19@korea.ac.kr
- Publication Type:Original Article
- Keywords:
cell hypoxia;
endothelial cells;
hydrogen peroxide;
hypoxia inducible factor 1, alpha subunit;
Wee1 protein, human
- MeSH:
Animals;
CDC2 Protein Kinase/metabolism;
Cell Cycle Proteins/*genetics/metabolism;
Cell Hypoxia;
Cell Line;
Cell Survival;
Endothelial Cells/cytology/*metabolism;
*Gene Expression Regulation;
Hydrogen Peroxide/*metabolism;
Mice;
Nuclear Proteins/*genetics/metabolism;
Pancreas/cytology;
Phosphorylation;
Protein-Tyrosine Kinases/*genetics/metabolism
- From:Experimental & Molecular Medicine
2011;43(12):653-659
- CountryRepublic of Korea
- Language:English
-
Abstract:
In an oxygen-depleted environment, endothelial cells initiate an adaptive pattern of synthesis, which may enable them to survive hypoxic crises. Using high-resolution two-dimensional gel electrophoresis in conjunction with mass spectroscopy, we obtained a 24 differential display of proteins in the pancreatic endothelial cell line, MS-1, at four time points following induction of hypoxia. The induction of Wee1 under hypoxia was confirmed both at the mRNA and protein levels. The phosphorylation of cell division cycle 2, which is downstream of Wee1, was also increased after hypoxic exposure. In addition, pre-exposure to hypoxia attenuated a decrease in hydrogen peroxide-induced cell number. The induction of bax (a pro-apoptotic protein) and reduction of bcl (an anti-apoptotic protein) after hypoxia stimulus were also attenuated by hypoxic pre-exposure. Moreover, hydrogen peroxide-induced morphologic damage did not appear in the wild-type Wee1-expressing cells. Taken together, our results suggest that Wee1 may have important role in hypoxia-induced pathophysiological situations in endothelial cells.
