Effects of ferulic acid on diabetic nephropathy in a rat model of type 2 diabetes.
10.3858/emm.2011.43.12.078
- Author:
Ran CHOI
1
;
Bo Hwan KIM
;
Jarinyaporn NAOWABOOT
;
Mi Young LEE
;
Mi Ri HYUN
;
Eun Ju CHO
;
Eun Soo LEE
;
Eun Young LEE
;
Young Chul YANG
;
Choon Hee CHUNG
Author Information
1. Department of Internal Medicine, Yonsei University Wonju College of Medicine, Wonju 220-701, Korea. cchung@yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
diabetes mellitus, experimental;
diabetic nephropathies;
ferulic acid;
inflammation;
oxidative stress
- MeSH:
Animals;
Anti-Inflammatory Agents, Non-Steroidal/*therapeutic use;
Antioxidants/*therapeutic use;
Cells, Cultured;
Chemokine CCL2/genetics/urine;
Collagen/analysis;
Coumaric Acids/*therapeutic use;
Diabetes Mellitus, Type 2/*complications;
Diabetic Nephropathies/*complications/*drug therapy/pathology;
Gene Expression/drug effects;
Kidney/drug effects/pathology;
Malondialdehyde/urine;
Podocytes/drug effects/metabolism;
Rats;
Rats, Inbred OLETF;
Reactive Oxygen Species/metabolism;
Transforming Growth Factor beta1/analysis
- From:Experimental & Molecular Medicine
2011;43(12):676-683
- CountryRepublic of Korea
- Language:English
-
Abstract:
Diabetic nephropathy is the most serious complication in diabetes mellitus. It is known that oxidative stress and inflammation play a central role in the development of diabetic nephropathy. In this study, we investigated that ferulic acid (FA) known as anti-oxidative agent could effect on diabetic nephropathy by anti-oxidative and anti-inflammatory mechanism. We examined the effects of FA in obese diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats and non-diabetic control Long-Evans Tokushima Otsuka (LETO) rats. We treated FA to experimental rats from 26 to 45 weeks of age. We evaluated ACR, MDA and MCP-1 in 24 h urine and examined renal histopathology and morphologic change in extracted kidneys from rats. Also, we evaluated the ROS production and MCP-1 levels in cultured podocyte after FA treatment. In the FA-treated OLETF rats, blood glucose was significantly decreased and serum adiponectin levels were increased. Urinary ACR was significantly reduced in FA-treated OLETF rats compared with diabetic OLETF rats. In renal histopathology, FA-treated OLETF rats showed decreased glomerular basement membrane thickness, glomerular volume, and mesangial matrix expansion. FA treatment decreased oxidative stress markers and MCP-1 levels in 24 h urine of rats and supernatants of cultured podocyte. In conclusion, it was suggested that FA have protective and therapeutic effects on diabetic nephropathy by reducing oxidative stress and inflammation.
