Early quantification of the therapeutic efficacy of the vascular disrupting agent, CKD-516, using dynamic contrast-enhanced ultrasonography in rabbit VX2 liver tumors.
- Author:
Ijin JOO
1
;
Jung Hoon KIM
;
Jeong Min LEE
;
Jin Woo CHOI
;
Joon Koo HAN
;
Byung Ihn CHOI
Author Information
1. Department of Radiology, Seoul National University Hospital, Seoul, Korea. jhkim2008@gmail.com
- Publication Type:Original Article
- Keywords:
Liver neoplasms;
Drug therapy;
CKD-516;
Ultrasonography;
Perfusion
- MeSH:
Animals;
Area Under Curve;
Drug Therapy;
Follow-Up Studies;
Hemodynamics;
Liver Neoplasms;
Liver*;
Perfusion;
Rabbits;
Ultrasonography*
- From:
Ultrasonography
2014;33(1):18-25
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To evaluate the usefulness of dynamic contrast-enhanced ultrasonography (DCE-US) in the early quantification of hemodynamic change following administration of the vascular disrupting agent (VDA) CKD-516 using a rabbit VX2 liver tumor model. METHODS: This study was approved by our institutional animal care and use committee. Eight VX2 liver-tumor-bearing rabbits were treated with intravenous CKD-516, and all underwent DCE-US using SonoVue before and again 2, 4, 6, and 24 hours following their treatment. The tumor perfusion parameters were obtained from the time-intensity curve of the DCE-US data. Repeated measures analysis of variance was performed to assess any significant change in tumor perfusion over time. Relative changes in the DCE-US parameters between the baseline and follow-up assessments were correlated with the relative changes in tumor size over the course of seven days using Pearson correlation. RESULTS: CKD-516 treatment resulted in significant changes in the DCE-US parameters, including the peak intensity, total area under the time-intensity curve (AUCtotal), and AUC during wash-out (AUCout) over time (P<0.05). Pairwise comparison tests revealed that the AUCtotal and AUC during wash-in (AUCin) seen on the two-hour follow-up were significantly lower than the baseline values (P<0.05). However, none of early changes in the DCE-US parameters until 24-hour follow-up showed a significant correlation with the relative changes in tumor size during seven days after CKD-516 treatment. CONCLUSION: Our results suggest that a novel VDA (CKD-516) can cause disruption of tumor perfusion as early as two hours after treatment and that the therapeutic effect of CKD-516 treatment can be effectively quantified using DCE-US.
