Improvement of Ergone Production from Mycelial Culture of Polyporus umbellatus.
- Author:
Wi Young LEE
1
;
Youngki PARK
;
Jin Kwon AHN
Author Information
1. Division of Biotechnology, Korea Forest Research Institute, Suwon 441-350, Korea.
- Publication Type:Original Article
- Keywords:
Armillariella mellea;
Co-culture;
Ergone;
Mycelial culture;
Polyporus umbellatus
- MeSH:
Agaricales;
Carbon;
Coculture Techniques;
Diuretics;
Fruit;
Hydrogen-Ion Concentration;
Mycelium;
Polyporus*
- From:Mycobiology
2007;35(2):82-86
- CountryRepublic of Korea
- Language:English
-
Abstract:
Ergone,a fungal metabolite derived from ergosterol,was previously isolated and identified from Polyporus umbellatus. Ergone is a major component of P. umbellatus known to have anti-aldosteronic diuretic effect and also displays cytotoxic activities. Most of mushroom's fruit bodies used for test contained less than 10 microg/g of ergone. But P. umbellatus have larger amount of ergone than any other mushrooms. In order to improve the ergone production from the submerged culture of P. umbellatus, several factors including medium composition,culture conditions (temperature and pH) and different combinations of co-cultivation with various mycelia were studied. Among various carbon sources examined,starch proved to be most effective for the production of mycelia. The optimum pH and temperature for a flask culture of P. umbellatus mycelia were found to be 4.5 and 25degrees C,respectively. Under the optimized culture conditions,both the ergone production (86.9 microg/g) and mycelial growth (3.5 g/l) increased when P. umbellatus was cultured with Armillariella mellea. When the optimized conditions were applied,both mycelium and ergone production were significantly enhanced.
