Production of the Acetylcholinesterase Inhibitor from Yarrowia lipolytica S-3.
- Author:
Dae Hyung LEE
1
;
Ji Su LEE
;
Sung Hun YI
;
Jong Soo LEE
Author Information
1. Department of Life Science and Genetic Engineering, Paichai University, Daejeon 302-735, Korea. biotech8@pcu.ac.kr
- Publication Type:Original Article
- Keywords:
Acetylcholinesterase inhibitor;
Antidementia;
Yarrowia lipolytica S-3
- MeSH:
Acetylcholinesterase;
Glucose;
Hydrogen-Ion Concentration;
Inhibitory Concentration 50;
Peptones;
Yarrowia;
Yeasts
- From:Mycobiology
2008;36(2):102-105
- CountryRepublic of Korea
- Language:English
-
Abstract:
The acetylcholinesterase (AChE) inhibitor of Yarrowia lipolytica S-3 was maximally produced when it was incubated at 30degrees C for 36 h in an optimal medium containing 1% yeast extract, 2% peptone and 2% glucose, with an initial pH 6.0. The final AChE inhibitory activity under these conditions was an IC50 value of 64 mg/ml. After partial purification of the AChE inhibitor by means of systematic solvent extraction, the final IC50 value of the partially purified AChE inhibitor was 0.75 mg/ml. We prepared a test product by using the partially purified AChE inhibitor and then determined its stability for the development of a new antidementia commercial product. The test product was stable at room temperature for 15 weeks.
