Flavone Biotransformation by Aspergillus niger and the Characterization of Two Newly Formed Metabolites.
- Author:
Yehia A MAHMOUD
1
;
Suzan W ASSAWAH
;
Saleh H EL-SHARKAWY
;
Amal ABDEL-SALAM
Author Information
1. Tanta University, Faculty of Science, Botany Department, Mycology Research Lab., Tanta 31527, Egypt. Yehiamah@gmail.com
- Publication Type:Original Article
- Keywords:
Anit-microbial;
Antioxidant;
Aspergillus;
Biotransformation;
Flavone
- MeSH:
Ascorbic Acid;
Aspergillus;
Aspergillus flavus;
Aspergillus niger;
Bacillus subtilis;
Biotransformation;
Candida albicans;
Escherichia coli;
Fermentation;
Flavones;
Fusarium;
Garlic;
Klebsiella;
Mass Spectrometry;
Pneumonia;
Pseudomonas aeruginosa;
Saccharomyces
- From:Mycobiology
2008;36(2):121-133
- CountryRepublic of Korea
- Language:English
-
Abstract:
Aspergillus niger isolated from Allium sativum was used at large scale fermentation (150 mg flavone/200 ml medium) to obtain suitable amounts of the products, efficient for identification. Then spectral analysis (UV, IR, 1H-NMR, 13C-NMR) and mass spectrometry were performed for the two products, which contributed to the identification process. The metabolite (1) was identified as 2'-hydroxydihydrochalcone, and the metabolite (2) was identified as 2'-hydroxyphenylmethylketone, which were more active than flavone itself. Antioxidant activities of the two isolated metabolites were tested compared with ascorbic acid. Antioxidant activity of metabolite (1) was recorded 64.58% which represented 79% of the antioxidant activity of ascorbic acid, and metabolite (2) was recorded 54.16% (67% of ascorbic acid activity). However, the antioxidant activity of flavone was recorded 37.50% which represented 46% of ascorbic acid activity. The transformed products of flavone have antimicrobial activity against Pseudomonas aeruginosa, Aspergillus flavus and Candida albicans, with MIC was recorded 250 microg/ml for metabolite (2) against all three organism and 500, 300, and 300 microg/ml for metabolite (1) against tested microorganisms (P. aeruginosa, Escherichia coli, Bacillus subtilis, and Klebsiella pneumonia, Fusarium moniliforme, A. flavus, Saccharomyces cerviceae, Kluveromyces lactis and C. albicans) at this order.
