Production of Xylanolytic Enzyme Complex from Aspergillus flavus using Agricultural Wastes.
- Author:
Jeong Dong KIM
1
Author Information
1. Department of Life Science, Hanyang University, Seoul 133-701, Korea. jdkim@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Agricultural wastes;
Aspergillus flavus;
Rice bran;
Xylanolytic enzyme
- MeSH:
Aspergillus flavus*;
Aspergillus*;
Carbon;
Electrophoresis, Polyacrylamide Gel;
Hordeum;
Hydrogen-Ion Concentration;
Zea mays
- From:Mycobiology
2005;33(2):84-89
- CountryRepublic of Korea
- Language:English
-
Abstract:
Five types of agricultural wastes were used for the production of xylanolytic enzyme by Aspergillus flavus K-03. All wastes materials supported high levels of xylanase and beta-xylosidase production. A high level of proteolytic activity was observed in barley and rice bran cultures, while only a weak proteolytic activity was detected in corn cob, barley and rice straw cultures. Maximum production of xylanase was achieved in basal liquid medium containing rice barn as carbon source for 5 days of culture at pH 6.5 and 25degrees C. The xylanolytic enzyme of A. flavus K-03 showed low thermostability. The times required for 50% reduction of the initial enzyme activity were 90 min at 40degrees C, 13 min at 50degrees C, and 3 min at 60degrees C. Xylanolytic activity showed the highest level at pH 5.5~10.5 and more than 70% of the original activity was retained at pH 6.5 and 7.0. The higher stability of xylanolytic enzymes in the broad range of alkaline pH is useful for utilization of the enzymes in industrial process requiring in alkaline conditions. Moreover, the highest production of xylanolytic enzyme was obtained when 0.5% of rice bran was supplied in basal liquid medium. SDS-PAGE analysis revealed a single xylanase band of approximately 28.5 kDa from the culture filtrates.
