Isolation of Fungal Pathogens to an Edible Mushroom, Pleurotus eryngii, and Development of Specific ITS Primers.
- Author:
Sang Woo KIM
1
;
Sinil KIM
;
Hyun Jun LEE
;
Ju Wan PARK
;
Hyeon Su RO
Author Information
1. Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University, Jinju 660-771, Korea. rohyeon@gnu.ac.kr
- Publication Type:Brief Communication
- Keywords:
Fungal pathogens;
ITS primer;
Mushroom;
Pleurotus eryngii
- MeSH:
Agaricales*;
Cladosporium;
DNA;
Fungi;
Mucor;
Pleurotus*;
Polymerase Chain Reaction;
Trichoderma
- From:Mycobiology
2013;41(4):252-255
- CountryRepublic of Korea
- Language:English
-
Abstract:
Fungal pathogens have caused severe damage to the commercial production of Pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. Four strains of pathogenic fungi, including Trichoderma koningiopsis DC3, Phomopsis sp. MP4, Mucor circinelloides MP5, and Cladosporium bruhnei MP6, were isolated from the bottle culture of diseased P. eryngii. A species-specific primer set was designed for each fungus from the ITS1-5.8S rDNA-ITS2 sequences. PCR using the ITS primer set yielded a unique DNA band for each fungus without any cross-reaction, proving the validity of our method in detection of mushroom fungal pathogens.
