An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides.
10.5941/MYCO.2017.45.4.434
- Author:
Hanyu ZHU
1
;
Xueyan SUN
;
Dongmei LIU
;
Liesheng ZHENG
;
Liguo CHEN
;
Aimin MA
Author Information
1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. aiminma@mail.hzau.edu.cn
- Publication Type:Brief Communication
- Keywords:
Dimorphism;
Jelly mushroom;
RNA extraction;
Tremella fuciformis
- MeSH:
Agaricales*;
Fungi;
Humans;
Methods*;
Mycelium;
Parents;
Polymerase Chain Reaction;
Polysaccharides*;
Real-Time Polymerase Chain Reaction;
RNA*
- From:Mycobiology
2017;45(4):434-437
- CountryRepublic of Korea
- Language:English
-
Abstract:
An improved method for extracting high quality and quantity RNA from a jelly mushroom and a dimorphic fungus—Tremella fuciformis which is especially rich in polysaccharides, is described. RNA was extracted from T. fuciformis mycelium M1332 and its parental monokaryotic yeast-like cells Y13 and Y32. The A260/280 and A260/230 ratios were both approximately 2, and the RNA integrity number was larger than 8.9. The yields of RNA were between 108 and 213 µg/g fresh wt. Downstream molecular applications including reverse transcriptional PCR and quantitative real-time PCR were also performed. This protocol is reliable and may be widely applicable for total RNA extraction from other jelly mushrooms or filamentous fungi rich in polysaccharides.
