Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae.
10.1080/12298093.2018.1454004
- Author:
Fang Jie YAO
1
;
Li Xin LU
;
Peng WANG
;
Ming FANG
;
You Min ZHANG
;
Ying CHEN
;
Wei Tong ZHANG
;
Xiang Hui KONG
;
Jia LU
;
Yoichi HONDA
Author Information
1. College of Horticulture, Jilin Agricultural University, Changchun, Jilin Province, PR China. yaofj@aliyun.com
- Publication Type:Original Article
- Keywords:
Auricularia auricula-judae;
fruiting body pattern;
sequence-related amplified polymorphism;
sequence characterized amplified region
- MeSH:
Breeding;
Chrysanthemum;
Cicatrix;
Clone Cells;
Fruit*;
Fungi
- From:Mycobiology
2018;46(1):72-78
- CountryRepublic of Korea
- Language:English
-
Abstract:
The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189–522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker “SCL-18” consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.
