Molecular Characterization of Fusarium solani and its Formae Speciales Based on Sequences Analysis of the Internal Transcribed Spacer (ITS) Region of Ribosomal DNA.
- Author:
Young Mi LEE
1
;
Yong Keel CHOI
;
Byung Re MIN
Author Information
1. Depament of Biology, Sangmyung University, Seoul 110-743, Korea.
- Publication Type:Original Article
- Keywords:
F. solani;
Formae speciales;
Internal transcribed spacer;
rDNA;
Sequencing
- MeSH:
Clinical Coding;
DNA, Ribosomal*;
Fusarium*;
RNA, Ribosomal, 5.8S
- From:Mycobiology
2000;28(2):82-88
- CountryRepublic of Korea
- Language:English
-
Abstract:
The sequences of the internal transcribed spacer (ITS) and 5.8s ribosomal RNA gene (rDNA) from Fusarium solani and its four formae speciales belonging to section Martiella was determined to investigate intraspecific divergence of the ITS regions. The length of the 5.8S, a coding region, was equally 158 bp at all isolates, whereas the variable range of ITS region was shown at 147~152 bp (ITS1) and 148~174 bp (ITS2). According to the maximum-matching method, the matching percentage was 94~100 at 5.8s rDNA, 77~97 at ITS1, and 67~97 at ITS2, respectively. In dendrogram based on the alignment of the ITS sequence data, F.solani f. sp. piperis was distinguished from other isolates belonging to the same species and nucleotide identity was considerably low (41.5%).
