Chemical Constituents of the Culture Broth of Phellinus linteus and Their Antioxidant Activity.
10.5941/MYCO.2015.43.1.43
- Author:
Myeong Seok LEE
1
;
Byung Soon HWANG
;
In Kyoung LEE
;
Geon Sik SEO
;
Bong Sik YUN
Author Information
1. Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea. bsyun@jbnu.ac.kr
- Publication Type:Original Article
- Keywords:
Antioxidant;
Caffeic acid;
Inotilone;
Medicinal fungus;
Phellinus linteus
- MeSH:
Chromatography;
Chromatography, Liquid;
Fungi;
Glucose;
Humans;
Medicine, Traditional;
Solanum tuberosum
- From:Mycobiology
2015;43(1):43-48
- CountryRepublic of Korea
- Language:English
-
Abstract:
The medicinal fungus Phellinus linteus, in the family Hymenochaetaceae, has been used as a traditional medicine for the treatment of various diseases. In this study, the chemical constituents of the culture broth of P. linteus were investigated. P. linteus was cultured in potato dextrose broth medium, and the culture broth was extracted with ethyl acetate. The ethyl acetate-soluble portion was concentrated and subjected to ODS column chromatography, followed by Sephadex LH-20 column chromatography. Six compounds (1~6) were purified by preparative reversed-phase high-performance liquid chromatography. Spectroscopic methods identified their structures as caffeic acid (1), inotilone (2), 4-(3,4-dihydroxyphenyl)-3-buten-2-one (3), phellilane H (4), (2E,4E)-(+)-4'-hydroxy-gamma-ionylideneacetic acid (5), and (2E,4E)-gamma-ionylideneacetic acid (6). Compounds 1, 2, and 3 exhibited potent dose-dependent antioxidant activity.
