Acetyl Eburicoic Acid from Laetiporus sulphureus var. miniatus Suppresses Inflammation in Murine Macrophage RAW 264.7 Cells.
10.5941/MYCO.2015.43.2.131
- Author:
Evelyn SABA
1
;
Youngmin SON
;
Bo Ra JEON
;
Seong Eun KIM
;
In Kyoung LEE
;
Bong Sik YUN
;
Man Hee RHEE
Author Information
1. Laboratory of Veterinary Physiology and Cell Signaling, Cardiovascular Research Institute and College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea. rheemh@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Acetyl eburicoic acid;
Anti-inflammation;
Laetiporus sulphureus var. miniatus;
Nitric oxide;
Proinflammatory cytokine
- MeSH:
Basidiomycota;
Cyclooxygenase 2;
Cytokines;
Fruit;
Fungi;
Glyceraldehyde 3-Phosphate;
Inflammation*;
Interleukin-6;
Interleukins;
Macrophages*;
Nitric Oxide;
Nitric Oxide Synthase Type II;
Oxidoreductases;
Polyporaceae;
Polyporales;
Polysaccharides;
Trees;
Tumor Necrosis Factor-alpha;
Tyramine
- From:Mycobiology
2015;43(2):131-136
- CountryRepublic of Korea
- Language:English
-
Abstract:
The basidiomycete Laetiporus sulphureus var. miniatus belongs to the Aphyllophorales, Polyporaceae, and grows on the needleleaf tree. The fruiting bodies of Laetiporus species are known to produce N-methylated tyramine derivatives, polysaccharides, and various lanostane triterpenoids. As part of our ongoing effort to discover biologically active compounds from wood-rotting fungi, an anti-inflammatory triterpene, LSM-H7, has been isolated from the fruiting body of L. sulphureus var. miniatus and identified as acetyl eburicoic acid. LSM-H7 dose-dependently inhibited the NO production in RAW 264.7 cells without any cytotoxicity at the tested concentrations. Furthermore it suppressed the production of proinflammatory cytokines, mainly inducible nitric oxide synthase, cyclooxygenase-2, interleukin (IL)-1beta, IL-6 and tumor necrosis factor alpha, when compared with glyceraldehyde 3-phosphate dehydrogenase. These data suggest that LSM-H7 is a crucial component for the anti-inflammatory activity of L. sulphureus var. miniatus.
