Isolation and Characterization of Cryptococcus neoformans from Environmental Sources in Busan.
- Author:
Kwang Seok OH
1
;
Soo Myung HWANG
Author Information
1. Maritime Safety Team, Korea Institute of Maritime and Fisheries Technology, Busan 608-080, Korea.
- Publication Type:Original Article
- Keywords:
API-ZYM;
Cryptococcus neoformans;
Pigeon droppings;
RAPD
- MeSH:
Busan*;
Columbidae;
Cryptococcus neoformans*;
Cryptococcus*;
DNA;
Soil
- From:Mycobiology
2005;33(4):188-193
- CountryRepublic of Korea
- Language:English
-
Abstract:
Twenty nine samples of pigeon droppings (n = 12) and soil contaminated with avian excreta (n = 19), collected from different sites in Busan, were examined for isolation and characterization of Cryptococcus neoformans. Of these samples, 5 strains of C. neoformans were recovered from pigeon droppings (5/12 : 41.7%). All isolates were belonged to C. neoformans var. grubii (serotype A). The extracellular enzyme activities of the strains by using the API-ZYM system showed two different enzymatic patterns. The genetic variability among C. neoformans isolates was analyzed by random amplified polymorphic DNA (RAPD) using three 10-mer primers. Two different RAPD patterns, which clearly distinguished the isolates, were identified. Analysis of RAPD patterns provided a good characterization of environmental strains of C. neoformans serotype A as a heterogeneous group and were in good agreement with enzymatic profiles.
