Molecular Marker Related to Fruitbody Color of Flammulina velutipes.
- Author:
Won Sik KONG
1
;
Chang Hyun YOU
;
Young Bok YOO
;
Gyu Hyun KIM
;
Kwang Ho KIM
Author Information
1. Applied Microbiology Division, National Institute of Agricultural Science & Technology, RDA, Suwon 441-707, Korea. wskong@rda.go.kr
- Publication Type:Original Article
- Keywords:
Backcross;
Bulked segregant analysis;
Flammulina velutipes;
Molecular marker
- MeSH:
Clone Cells;
Flammulina*;
Genes, vif
- From:Mycobiology
2004;32(1):6-10
- CountryRepublic of Korea
- Language:English
-
Abstract:
White and brown strains of Flammulina velutipes were inter-crossed. All F1 showed light-brown fruitbody, suggesting that a gene for the brown fruitbody was incompletely dominant against the white one. And backcross experiment showed that more than two genes were involved in color determination. To isolate a molecular marker linked to fruitbody color, a set of primers was designed from a sequence of clones derived by a bulked segregant analysis. These markers showed a specific band which co-segregated with brown fruitbody forming strains.