DNA Profiles of Trichoderma spp. in Korea.
- Author:
Dong Suk PARK
1
;
Hee Wan KANG
;
Young Jin PARK
;
Mi Hee LEE
;
Byoung Moo LEE
;
Jang Ho HAHN
;
Seung Joo GO
Author Information
1. Bioinformatics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea. dspark@rda.go.kr
- Publication Type:Original Article
- Keywords:
DNA fingerprinting;
Internal transcribed spacer (ITS);
Trichoderma;
URP-PCR
- MeSH:
Agaricales;
Classification;
DNA Fingerprinting;
DNA*;
DNA, Ribosomal;
Genetic Variation;
Korea*;
Sequence Alignment;
Trichoderma*
- From:Mycobiology
2004;32(1):24-34
- CountryRepublic of Korea
- Language:English
-
Abstract:
Molecular approaches, internal transcribed spacer(ITS) sequences of ribosomal DNA, and Universal Rice Primer Polymerase Chain Reaction(URP-PCR) were used to investigate the genetic diversity, taxonomic complexity, and relationships of Trichoderma species in mushroom farms. Forty-one isolates of 13 Trichoderma spp. were used in this study and clustered into eight groups. The DNA fingerprint patterns and ITS1 region sequence alignment data showed similar results, but not in some species, such as T. virens, T. atroviride, T. harzianum, and T. aureoviride. Results of this study have proven that the morphology-based taxonomic system has some limitations in terms of classification. The data obtained in this study would be a good index for classifying indistinguishable Trichoderma strains.
