LAMMER Kinase Lkh1 Is an Upstream Regulator of Prk1-Mediated Non-Sexual Flocculation in Fission Yeast.
10.1080/12298093.2018.1513115
- Author:
Yoon Dong PARK
1
;
Soo Jeong KWON
;
Kyung Sook BAE
;
Hee Moon PARK
Author Information
1. Department of Microbiology & Molecular Biology, Chungnam National University, Daejeon, Korea. hmpark@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Non-sexual flocculation;
LAMMER kinase;
Lkh1;
Prk1;
negative regulator;
Schizosaccharomyces pombe
- MeSH:
Flocculation*;
Phenotype;
Phosphotransferases*;
Schizosaccharomyces*
- From:Mycobiology
2018;46(3):236-241
- CountryRepublic of Korea
- Language:English
-
Abstract:
The cation-dependent galactose-specific flocculation activity of the Schizosaccharomyces pombe null mutant of lkh1⁺, the gene encoding LAMMER kinase homolog, has previously been reported by our group. Here, we show that disruption of prk1⁺, another flocculation associated regulatory kinase encoding gene, also resulted in cation-dependent galactose-specific flocculation. Deletion of prk1 increased the flocculation phenotype of the lkh1⁺ null mutant and its overexpression reversed the flocculation of cells caused by lkh1 deletion. Transcript levels of prk1⁺ were also decreased by lkh1⁺ deletion. Cumulatively, these results indicate that Lkh1 is one of the negative regulators acting upstream of Prk1, regulating non-sexual flocculation in fission yeast.
