Depletion of epsilon-COP in the COPI Vesicular Coat Reduces Cleistothecium Production in Aspergillus nidulans.
10.5941/MYCO.2015.43.1.31
- Author:
Eun Hye KANG
1
;
Eun Jung SONG
;
Jun Ho KOOK
;
Hwan Hee LEE
;
Bo Ri JEONG
;
Hee Moon PARK
Author Information
1. Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-764, Korea. hmpark@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
alpha-COP;
Aspergillus nidulans;
Cleistothecium;
epsilon-COP;
Sexual development
- MeSH:
Aspergillus nidulans*;
Coatomer Protein*;
Fruit;
Homologous Recombination;
Mass Screening;
Saccharomyces cerevisiae;
Saccharomycetales;
Sexual Development;
Yeasts
- From:Mycobiology
2015;43(1):31-36
- CountryRepublic of Korea
- Language:English
-
Abstract:
We have previously isolated epsilon-COP, the alpha-COP interactor in COPI of Aspergillus nidulans, by yeast two-hybrid screening. To understand the function of epsilon-COP, the aneA+ gene for epsilon-COP/AneA was deleted by homologous recombination using a gene-specific disruption cassette. Deletion of the epsilon-COP gene showed no detectable changes in vegetative growth or asexual development, but resulted in decrease in the production of the fruiting body, cleistothecium, under conditions favorable for sexual development. Unlike in the budding yeast Saccharomyces cerevisiae, in A. nidulans, over-expression of epsilon-COP did not rescue the thermo-sensitive growth defect of the alpha-COP mutant at 42degrees C. Together, these data show that epsilon-COP is not essential for viability, but it plays a role in fruiting body formation in A. nidulans.
