Viral Effects of a dsRNA Mycovirus (PoV-ASI2792) on the Vegetative Growth of the Edible Mushroom Pleurotus ostreatus.
10.5941/MYCO.2016.44.4.283
- Author:
Ha Yeon SONG
1
;
Hyo Jin CHOI
;
Hansaem JEONG
;
Dahye CHOI
;
Dae Hyuk KIM
;
Jung Mi KIM
Author Information
1. Department of Bio-Environmental Chemistry, Institute of Life Science and Natural Resources, Wonkwang University, Iksan 54538, Korea. micro@wku.ac.kr
- Publication Type:Original Article
- Keywords:
Curing;
Isogenic strain;
Mycovirus;
Pleurotus ostreatus;
Viral symptom
- MeSH:
Agar;
Agaricales*;
Blotting, Northern;
Clone Cells;
Culture Media;
DNA, Complementary;
Electrophoresis;
Fruit;
Fungal Viruses*;
Methods;
Pleurotus*;
RNA Replicase;
RNA, Double-Stranded;
Weights and Measures
- From:Mycobiology
2016;44(4):283-290
- CountryRepublic of Korea
- Language:English
-
Abstract:
A double-stranded RNA (dsRNA) mycovirus was detected in malformed fruiting bodies of Pleurotus ostreatus strain ASI2792, one of bottle cultivated commercial strains of the edible oyster mushroom. The partial RNA-dependent RNA polymerase (RdRp) gene of the P. ostreatus ASI2792 mycovirus (PoV-ASI2792) was cloned, and a cDNA sequences alignment revealed that the sequence was identical to the RdRp gene of a known PoSV found in the P. ostreatus strain. To investigate the symptoms of PoV-ASI2792 infection by comparing the isogenic virus-free P. ostreatus strains with a virus-infected strain, isogenic virus-cured P. ostreatus strains were obtained by the mycelial fragmentation method for virus curing. The absence of virus was verified with gel electrophoresis after dsRNA-specific virus purification and Northern blot analysis using a partial RdRp cDNA of PoV-ASI2792. The growth rate and mycelial dry weight of virus-infected P. ostreatus strain with PoV-ASI2792 mycovirus were compared to those of three virus-free isogenic strains on 10 different media. The virus-cured strains showed distinctly higher mycelial growth rates and dry weights on all kinds of experimental culture media, with at least a 2.2-fold higher mycelial growth rate on mushroom complete media (MCM) and Hamada media, and a 2.7-fold higher mycelial dry weight on MCM and yeastmalt-glucose agar media than those of the virus-infected strain. These results suggest that the infection of PoV mycovirus has a deleterious effect on the vegetative growth of P. ostreatus.
