Evaluation of Antioxidant, Anti-cholinesterase, and Anti-inflammatory Effects of Culinary Mushroom Pleurotus pulmonarius.
10.5941/MYCO.2016.44.4.291
- Author:
Trung Kien NGUYEN
1
;
Kyung Hoan IM
;
Jaehyuk CHOI
;
Pyung Gyun SHIN
;
Tae Soo LEE
Author Information
1. Division of Life Sciences, Incheon National University, Incheon 22012, Korea. tslee@incheon.ac.kr
- Publication Type:Original Article
- Keywords:
Anti-cholinesterase;
Anti-inflammation;
Antioxidant;
Cytotoxicity;
Pleurotus pulmonarius
- MeSH:
Acetylcholinesterase;
Agaricales*;
Asian Continental Ancestry Group;
Butylated Hydroxytoluene;
Butyrylcholinesterase;
Cholinesterases;
Chromatography, Liquid;
Edema;
Fruit;
Galantamine;
Glutamic Acid;
Humans;
Hydroxyl Radical;
Inflammation;
Macrophages;
Methanol;
Models, Animal;
Nitric Oxide;
Nitric Oxide Synthase Type II;
Phenol;
Pleurotus*
- From:Mycobiology
2016;44(4):291-301
- CountryRepublic of Korea
- Language:English
-
Abstract:
Culinary mushroom Pleurotus pulmonarius has been popular in Asian countries. In this study, the anti-oxidant, cholinesterase, and inflammation inhibitory activities of methanol extract (ME) of fruiting bodies of P. pulmonarius were evaluted. The 1,1-diphenyl-2-picryl-hydrazy free radical scavenging activity of ME at 2.0 mg/mL was comparable to that of butylated hydroxytoluene, the standard reference. The ME exhibited significantly higher hydroxyl radical scavenging activity than butylated hydroxytoluene. ME showed slightly lower but moderate inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase than galantamine, a standard AChE inhibitor. It also exhibited protective effect against cytotoxicity to PC-12 cells induced by glutamate (10~100 µg/mL), inhibitory effect on nitric oxide (NO) production and inducible nitric oxide synthase protein expression in lipopolysaccharide-stimulated RAW 264.7 macrophages, and carrageenan-induced paw edema in a rat model. High-performance liquid chromatography analysis revealed the ME of P. pulmonarius contained at least 10 phenolic compounds and some of them were identified by the comparison with known standard phenolics. Taken together, our results demonstrate that fruiting bodies of P. pulmonarius possess antioxidant, anti-cholinesterase, and inflammation inhibitory activities.
