Cloning and Sequence Analysis of the Cellobiohydrolase I Genes from Some Basidiomycetes.
10.5941/MYCO.2012.40.2.107
- Author:
Ekachai CHUKEATIROTE
1
;
Sajeewa S N MAHARACHCHIKUMBURA
;
Shannaphimon WONGKHAM
;
Phongeun SYSOUPHANTHONG
;
Rungtiwa PHOOKAMSAK
;
Kevin D HYDE
Author Information
1. Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. ekachai@mfu.ac.th
- Publication Type:Original Article
- Keywords:
Basidiomycetes;
cbh;
Cellobiohydrolase;
Cellulase;
Gene cloning
- MeSH:
Amino Acid Sequence;
Base Sequence;
Basidiomycota;
Bromides;
Cellulase;
Cellulose 1,4-beta-Cellobiosidase;
Clinical Coding;
Clone Cells;
Cloning, Organism;
DNA;
Humans;
Introns;
Pleurotus;
Polymerase Chain Reaction;
Quaternary Ammonium Compounds;
Sequence Analysis
- From:Mycobiology
2012;40(2):107-110
- CountryRepublic of Korea
- Language:English
-
Abstract:
Genes encoding the cellobiohydrolase enzyme (CBHI), designated as cbhI, were isolated from the basidiomycetes Auricularia fuscosuccinea, Pleurotus giganteus, P. eryngii, P. ostreatus, and P. sajor-caju. Initially, the fungal genomic DNA was extracted using a modified cetyltrimethyl ammonium bromide (CTAB) protocol and used as a DNA template. The cbhI genes were then amplified and cloned using the pGEM-T Easy Vector Systems. The sizes of these PCR amplicons were between 700~800 bp. The DNA sequences obtained were similar showing high identity to the cbhI gene family. These cbhI genes were partial consisting of three coding regions and two introns. The deduced amino acid sequences exhibited significant similarity to those of fungal CBHI enzymes belonging to glycosyl hydrolase family 7.
