Neuraminidase Inhibitors from the Culture Broth of Phellinus linteus.
10.5941/MYCO.2012.40.2.142
- Author:
Ji Hee YEOM
1
;
In Kyoung LEE
;
Dae Won KI
;
Myeong Seok LEE
;
Soon Ja SEOK
;
Bong Sik YUN
Author Information
1. Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea. bsyun@jbnu.ac.kr
- Publication Type:Brief Communication
- Keywords:
Hispidin;
Hypholomine B;
Neuraminidase inhibitor;
Phellinus linteus
- MeSH:
Chromatography;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Dextrans;
Fungi;
Inhibitory Concentration 50;
Neuraminidase;
Pyrones;
Retention (Psychology)
- From:Mycobiology
2012;40(2):142-144
- CountryRepublic of Korea
- Language:English
-
Abstract:
During the search for neuraminidase inhibitors from medicinal fungi, we found that the culture broth of Phellinus linteus exhibited potent inhibitory activity. Solvent partition, Sephadex LH-20 column chromatography, and high-performance liquid chromatography (HPLC) were performed for purification of two active substances from the culture broth. According to 1H NMR measurements and comparison of HPLC retention times with those of authentic compounds, their chemical structures were identified as hispidin and hypholomine B. Compounds (hispidin) 1 and 2 (hypholomine B) inhibited neuraminidase, with IC50 values of 13.1 and 0.03 microM, respectively.
