Biocontrol Activity of Volatile-Producing Bacillus megaterium and Pseudomonas protegens against Aspergillus flavus and Aflatoxin Production on Stored Rice Grains.
10.5941/MYCO.2017.45.3.213
- Author:
Mohamed MANNAA
1
;
Ji Yeon OH
;
Ki Deok KIM
Author Information
1. Laboratory of Plant Disease and Biocontrol, Department of Biosystems and Biotechnology, Korea University, Seoul 02841, Korea. kidkim@korea.ac.kr
- Publication Type:Original Article
- Keywords:
Aflatoxin;
Aspergillus flavus;
Bacillus megaterium;
Bacterial volatiles;
Pseudomonas protegens
- MeSH:
Aflatoxins*;
Agar;
Aspergillus flavus*;
Aspergillus*;
Bacillus megaterium*;
Bacillus*;
Germination;
Pseudomonas*;
Sphingomonas
- From:Mycobiology
2017;45(3):213-219
- CountryRepublic of Korea
- Language:English
-
Abstract:
In our previous study, three bacterial strains, Bacillus megaterium KU143, Microbacterium testaceum KU313, and Pseudomonas protegens AS15, were selected as effective biocontrol agents against Aspergillus flavus on stored rice grains. In this study, we evaluated the inhibitory effects of the volatiles produced by the strains on A. flavus growth and aflatoxin production on stored rice grains. The three strains significantly reduced mycelial growth of A. flavus in dual-culture assays compared with the negative control strain, Sphingomonas aquatilis KU408, and an untreated control. Of these tested strains, volatiles produced by B. megaterium KU143 and P. protegens AS15 markedly inhibited mycelial growth, sporulation, and conidial germination of A. flavus on agar medium and suppressed the fungal populations in rice grains. Moreover, volatiles produced by these two strains significantly reduced aflatoxin production in the rice grains by A. flavus. To our knowledge, this is the first report of the suppression of A. flavus aflatoxin production in rice grains using B. megaterium and P. protegens volatiles.
