Antifungical Activity of Autochthonous Bacillus subtilis Isolated from Prosopis juliflora against Phytopathogenic Fungi.
10.5941/MYCO.2017.45.4.385
- Author:
Ali ABDELMOTELEB
1
;
Rosalba TRONCOSO-ROJAS
;
Tania GONZALEZ-SOTO
;
Daniel GONZÁLEZ-MENDOZA
Author Information
1. Instituto de Ciencias Agrícolas de la Universidad Autónoma de Baja California (ICA-UABC), CP 21705, Ejido Nuevo León, Baja California, Mexico. daniasaf@gmail.com
- Publication Type:Original Article
- Keywords:
Bacillus subtilis;
Biocontrol;
Lipopeptides;
Mycolytic enzymes;
Prosopis juliflora
- MeSH:
Alternaria;
Bacillus subtilis*;
Bacillus*;
Botrytis;
Chitinase;
Colletotrichum;
Fungi*;
Lipopeptides;
Prosopis*
- From:Mycobiology
2017;45(4):385-391
- CountryRepublic of Korea
- Language:English
-
Abstract:
The ability of Bacillus subtilis, strain ALICA to produce three mycolytic enzymes (chitinase, β-1,3-glucanase, and protease), was carried out by the chemical standard methods. Bacillus subtilis ALICA was screened based on their antifungal activity in dual plate assay and cell-free culture filtrate (25%) against five different phytopathogenic fungi Alternaria alternata, Macrophomina sp., Colletotrichum gloeosporioides, Botrytis cinerea, and Sclerotium rolfesii. The B. subtilis ALICA detected positive for chitinase, β-1,3-glucanase and protease enzymes. Fungal growth inhibition by both strain ALICA and its cell-free culture filtrate ranged from 51.36% to 86.3% and 38.43% to 68.6%, respectively. Moreover, hyphal morphological changes like damage, broken, swelling, distortions abnormal morphology were observed. Genes expression of protease, β-1,3-glucanase, and lipopeptides (subtilosin and subtilisin) were confirmed their presence in the supernatant of strain ALICA. Our findings indicated that strain ALICA provided a broad spectrum of antifungal activities against various phytopathogenic fungi and may be a potential effective alternative to chemical fungicides.
