Methyl 9-Oxo-(10E,12E)-octadecadienoate Isolated from Fomes fomentarius Attenuates Lipopolysaccharide-Induced Inflammatory Response by Blocking Phosphorylation of STAT3 in Murine Macrophages.
10.5941/MYCO.2015.43.3.319
- Author:
Ji Hyun CHOE
1
;
Young Joo YI
;
Myeong Seok LEE
;
Dong Won SEO
;
Bong Sik YUN
;
Sang Myeong LEE
Author Information
1. Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental & Bioresources, Chonbuk National University, Iksan 54596, Korea. leesangm@jbnu.ac.kr bsyun@jbnu.ac.kr
- Publication Type:Original Article
- Keywords:
Anti-inflammatory effect;
Fomes fomentarius;
Macrophages;
STAT3
- MeSH:
Coriolaceae*;
Cyclooxygenase 2;
Cytokines;
Dinoprostone;
Down-Regulation;
Fungi;
Humans;
Interleukin-6;
Macrophages*;
Nitric Oxide;
Nitric Oxide Synthase;
Phosphorylation*;
Phosphotransferases;
Polyporaceae;
STAT3 Transcription Factor;
Tumor Necrosis Factor-alpha
- From:Mycobiology
2015;43(3):319-326
- CountryRepublic of Korea
- Language:English
-
Abstract:
Fomes fomentarius is a fungus of the Polyporaceae family and is used in traditional oriental therapies. Although the anti-inflammatory activities of this species have been previously reported, the identity of the bioactive compounds responsible for this activity remains unknown. Here, we investigated whether methyl 9-oxo-(10E,12E)-octadecadienoate (FF-8) purified from F. fomentarius exerts anti-inflammatory activity in murine macrophages stimulated with lipopolysaccharide (LPS). FF-8 suppressed secretion of nitric oxide (NO) and prostaglandin E2 through downregulation of inducible NO synthase and cyclooxygenase-2 expression induced by LPS. In addition, pretreatment of cells with FF-8 led to a reduction in levels of secreted inflammatory cytokines such as tumor necrosis factor-alpha and interleukin-6 in macrophages stimulated with LPS. Conversely, FF-8 did not affect nuclear factor kappaB, p38, c-Jun NH2-terminal kinase, and extracellular signal-regulated kinase pathways. Instead, FF-8 specifically interfered with signal transducer and activator of transcription 3 (STAT3) phosphorylation induced by LPS. Collectively, this study demonstrated that FF-8 purified from F. fomentarius suppresses inflammatory responses in macrophages stimulated with LPS by inhibiting STAT3 activation. Further studies will be required to elucidate the anti-inflammatory effect of FF-8 in vivo.
