Production of Aerial Conidia of Lecanicillium lecanii 41185 by Solid-State Fermentation for Use as a Mycoinsecticide.
10.4489/MYCO.2008.36.3.183
- Author:
Van Hanh VU
1
;
Suk Il HONG
;
Keun KIM
Author Information
1. Department of Bioscience and Biotechnology, The University of Suwon 445-743, Korea. kkim@suwon.ac.kr
- Publication Type:Original Article
- Keywords:
Aerial conidia;
Lecanicillium lecanii;
Mycoinsecticide;
Optimum condition;
Solid-state fermentation
- MeSH:
Aphids;
Fermentation;
Fungi;
Humidity;
Hydrogen-Ion Concentration;
Seeds;
Spores, Fungal;
Steam
- From:Mycobiology
2008;36(3):183-189
- CountryRepublic of Korea
- Language:English
-
Abstract:
The production of aerial conidia of Lecanicillium lecanii 41185, a highly virulent fungus, by solid-state fermentation was studied for use as a biocontrol agent against aphids. Among several agro-industrial solid media, steamed polished rice was found to produce the highest amount of aerial conidia. The optimal conditions for aerial conidia production were determined to be a 28.5% moisture content in the rice, 25degrees C culture temperature, rice pH of 6.0, 75% ambient relative humidity, 4-dold seeding culture, 0.6% KNO3, and 12 d of culture time. The conidia yield increased from 5.7 x 10(9) conidia/g polished rice to 18.2 x 10(9) conidia/g polished rice following application of these optimized conditions.