Effect of Chitosan Acetate on Bacteria Occurring on Neungee Mushrooms, Sarcodon aspratus.
10.4489/MYCO.2008.36.4.249
- Author:
Bom Soo PARK
1
;
Chang Duck KOO
;
Kang Hyeon KA
;
Young Nam LEE
Author Information
1. Division of Life Science, Chungbuk National University, Cheongju 361-763, Korea. ynlee@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Antibacterial activity;
Chitosan acetate;
Heterotropic bacteria;
Minimal inhibitory concentration (MIC);
Neungee mushroom;
Sarcodon aspratus
- MeSH:
Agaricales;
Bacteria;
Burkholderia cepacia complex;
Carbon;
Cellular Structures;
Chitosan;
Electrons;
Sprains and Strains
- From:Mycobiology
2008;36(4):249-254
- CountryRepublic of Korea
- Language:English
-
Abstract:
Minimal growth inhibitory concentrations (MICs) of chitosan acetate (M.W. 60 kDa) on heterotrophic bacteria (strains MK1, S, and R) isolated from the soft-rotten tissues of Neungee mushroom (Sarcodon aspratus) were measured. The slimy substance produced by the MK1 strain was responsible for the diseased mushroom's appearance. The S and R strains were members of the Burkholderia cepacia complex. These strains showed different levels of susceptibility toward chitosan acetate. The MIC of chitosan acetate against the MK1 and S strains was 0.06%. The MIC against the R strain was greater than 0.10%. Survival fractions of the MK1 and S strains at the MIC were 3 x 10(-4) and 1.4 x 10(-3) after 24 h, and 2 x 10(-4) and 7 x 10(-4) after 48 h, respectively. Survival fractions of the R strain after 24 and 48 hr at 0.1% chitosan acetate were 1 x 10-2 and 6.9 x 10(-3), respectively. Compared to the MK1 and S strains, the low susceptibility of the R stain towards chitosan acetate could be due to the ability of the R strain to utilize chitosan as a carbon source. Thirty-eight percent of Neungee pieces treated in a 0.06% chitosan acetate solution for 2~3 second did not show any bacterial growth at 4 days, whereas bacterial growth around untreated mushroom pieces occurred within 2 days. These data suggest that chitosan acetate is highly effective in controlling growth of indigenous microorganisms on Neungee. The scanning electron micrographs of the MK1 strain treated with chitosan revealed a higher degree of disintegrated and distorted cellular structures.
