Diversity and Plant Growth Promoting Capacity of Endophytic Fungi Associated with Halophytic Plants from the West Coast of Korea.
10.5941/MYCO.2015.43.4.373
- Author:
Irina KHALMURATOVA
1
;
Hyun KIM
;
Yoon Jong NAM
;
Yoosun OH
;
Min Ji JEONG
;
Hye Rim CHOI
;
Young Hyun YOU
;
Yeon Sik CHOO
;
In Jung LEE
;
Jae Ho SHIN
;
Hyeokjun YOON
;
Jong Guk KIM
Author Information
1. School of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University, Daegu 41566, Korea. kimjg@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Fungal endophytes;
Genetic diversity;
Gibberellin;
Halophytic plants;
Plant growth promotion;
Salt marsh
- MeSH:
Alternaria;
Ascomycota;
Basidiomycota;
Chenopodiaceae;
Endophytes;
Fungi*;
Fusarium;
Genetic Variation;
Gibberella;
Gibberellins;
Korea*;
Penicillium;
Plants*;
Plumbaginaceae;
Salt-Tolerant Plants*;
Seedlings;
Talaromyces;
Wetlands
- From:Mycobiology
2015;43(4):373-383
- CountryRepublic of Korea
- Language:English
-
Abstract:
Five halophytic plant species, Suaeda maritima, Limonium tetragonum, Suaeda australis, Phragmites australis, and Suaeda glauca Bunge, which are native to the Muan salt marsh of South Korea, were examined for fungal endophytes by sequencing the internal transcribed spacer (ITS) region containing ITS1, 5.8S rRNA, and ITS2. In total, 160 endophytic fungal strains were isolated and identified from the roots of the 5 plant species. Taxonomically, all 160 strains belonged to the phyla Ascomycota, Basidiomycota, and Zygomycota. The most dominant genus was Fusarium, followed by the genera Penicillium and Alternaria. Subsequently, using 5 statistical methods, the diversity indices of the endophytes were determined at genus level. Among these halophytic plants, P. australis was found to host the greatest diversity of endophytic fungi. Culture filtrates of endophytic fungi were treated to Waito-C rice seedlings for plant growth-promoting effects. The fungal strain Su-3-4-3 isolated from S. glauca Bunge provide the maximum plant length (20.1 cm) in comparison with wild-type Gibberella fujikuroi (19.6 cm). Consequently, chromatographic analysis of the culture filtrate of Su-3-4-3 showed the presence of physiologically active gibberellins, GA(1) (0.465 ng/mL), GA(3) (1.808 ng/mL) along with other physiologically inactive GA(9) (0.054 ng/mL) and GA(24) (0.044 ng/mL). The fungal isolate Su-3-4-3 was identified as Talaromyces pinophilus.
