Screening of Cell Cycle-Related Genes of Pleurotus eryngii Using Yeast Mutant Strains.
- Author:
Shanliang SHI
1
;
Hyeon Su RO
Author Information
1. Department of Microbiology and Research Institute of Life Science, Gyeongsang National University, Chinju 660-701, Korea. rohyeon@gnu.ac.kr
- Publication Type:Brief Communication
- Keywords:
Cell cycle;
Genomic DNA library;
Pleurotus eryngii;
Temperature sensitive;
Yeast
- MeSH:
Agaricales;
Base Sequence;
Cell Cycle;
DNA;
Gene Library;
Mass Screening;
Plasmids;
Pleurotus;
Saccharomyces cerevisiae;
Yeasts
- From:Mycobiology
2010;38(1):70-73
- CountryRepublic of Korea
- Language:English
-
Abstract:
Temperature-sensitive yeast mutants were used to screen for cell cycle-related genes from Pleurotus eryngii genomic DNA. A mushroom genomic DNA library was established and each gene was screened for the ability to rescue seven Saccharomyces cerevisiae temperature-sensitive strains. Hundreds of yeast transformants were selected at restrictive temperatures over 30degrees C. Plasmids from the transformants that survived were isolated and transformed back into their host strains. The temperature sensitivity of the resulting transformants was tested from 30degrees C to 37degrees C. Ten DNA fragments from P. eryngii were able to rescue yeast temperature-sensitive strains, and their DNA sequences were determined.
