Development of Detection Methods for Cellulolytic Activity of Auricularia auricula-judae.
- Author:
Woo Sik JO
1
;
Soon Hwa BAE
;
Seung Yong CHOI
;
So Deuk PARK
;
Young Bok YOO
;
Seung Chun PARK
Author Information
1. Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu 702-320, Korea. jowoosik@yahoo.co.kr
- Publication Type:Brief Communication
- Keywords:
Auricularia auricula-judae;
Cellulolytic enzyme;
Chromogenic media;
Congo red
- MeSH:
Benzenesulfonates;
Carbon;
Cellulose;
Congo Red;
Diminazene;
Hydrogen-Ion Concentration;
Phenolsulfonphthalein
- From:Mycobiology
2010;38(1):74-77
- CountryRepublic of Korea
- Language:English
-
Abstract:
To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from 15~35degrees C. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at 25degrees C for 5 days, the clear zone indicative of cellulolytic activity was more pronounced.
